Given the central metabolic function of mitochondria, it is not surprising that mitochondrial dynamics and bioenergetics reciprocally influence each other. Modeling mitochondrial bioenergetics with integrated volume dynamics jason n. Pdf mitochondrial bioenergetics, redox state, dynamics. The purpose of this special issue on mitochondrial dynamics. We are interested in how certain drugs and different types of dietary fats affect mitochondrial content and metabolism. Dysfunctional mitochondrial bioenergetics and oxidative.
Mitochondrial dysfunction and mitochondrial dynamicsthe. Professor saks has authored over 220 scientific publications and has received numerous scientific awards, including the ussr state prize in science and the internatinoal research and. Mitochondrial dynamics are generally associated with quality control, maintaining the functionality of bioenergetics. Bba bioenergetics covers the area of biological membranes involved in energy transfer and conversion. Metabolic regulation of mitochondrial dynamics journal of cell. Among phospholipid species of the inner mitochondrial membrane imm, cardiolipin cl is of particular importance because of its key role in mitochondrial bioenergetics 88, 91, 93, 106, in mitochondrial dynamics and quality control through fission and fusion 9, 34, 59, in mitophagy to remove damaged mitochondria, as well as in the. One can appreciate that the life cycle of mitochondria would be compromised if mitochondrial fusion or fission were disabled to allow for bioenergetic adaptation. Regulation of autophagy, mitochondrial dynamics, and.
In this report, we analyze the bidirectional relationships between mitochondrial morphology and function in living human cells. During the last decade, substantial empirical and in silico evidence was presented demonstrating that mitochondrial dynamics impacts on mitochondrial function and vice versa. Thirty years of progress in mitochondrial bioenergetics and. Mitochondrial dynamics in cardiovascular medicine gaetano. This text covers the basic principles of mitochondrial dynamics in cardiovascular medicine, with particular emphasis on their functional roles in physiology and disease. The lab is equipped with last generation oroboros oxygraph o2k instruments. Structural organization and dynamics of mitochondria in the cells in vivo. Mitochondrial bioenergetics and quality control mechanisms. Until now, one of the limitations in the research on mitochondrial dynamics, especially in mammals, has been the lack of a precise and reliable assay to quantify mitochondrial fusion and fission. Pdf relationships between mitochondrial dynamics and. In this issue of bmc biology, schauss and colleagues describe a simple, reliable, and robust novel assay that allows fusion of mammalian mitochondria to be quantified in vitro.
Rundell1 1weldon school of biomedical engineering, purdue university, west lafayette, indiana, united states of america, 2department of mathematics, purdue university, west. Chapter 2 relationships between mitochondrial dynamics and. Sep 19, 2007 a graduate of moscow university, he was the longtime head of the laboratory of cardiac bioenergetics in moscow, russia, before taking up his present positions. Developmental programming of mitochondrial biology. These findings indicate that highly reactive lipid species, such as 4hne, have specific targets in the both the mitochondria and autophagy pathway, with modification possibly affecting autophagy initiation, mitochondrial dynamics, and bioenergetics in a concentrationdependent manner, and that their combined effects are responsible for. Research on mechanisms underlying the phenomenon of developmental programming of health and disease has focused primarily on processes that are specific to cell types, organs and phenotypes of inte. Mitochondria are at the center of cellular energy metabolism and regulate cell life and death. Research team bioenergetics and dynamics of mitochondria. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs.
Calcium is an important signaling molecule involved in the regulation of many cellular functions. In particular, it focuses on the structures obtained by xray crystallography and other approaches, and molecular mechanisms of the components of photosynthesis, mitochondrial and bacterial respiration, oxidative phosphorylation, motility and. The equilibrium between mitochondrial fusionfission, as well as the organelle motility, determine network morphology and ultimately mitochondrialcell function. Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemicalinduced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.
These findings are demonstrated the handinhand nature of mitochondrial fissionfusion structural dynamics with bioenergetic function, with mtdna arrayed. Mitochondria act as the hubs of bioenergetics and metabolism. We also develop new tools to investigate interorganelar communication. Mitochondrial dynamics is a delicate balance between division and fusion. Dimers of mitochondrial atp synthase form the permeability transition pore.
Metabolic regulation of mitochondrial dynamics journal. The book is full of stepbystep how to methods with sample results, interpretations, and pitfalls. Mitochondria continuously adjust their architecture, position and overall fitness in response to cellular and extracellular cues figure 1. Analysis of mitochondrial dynamics and functions using. Mitochondria are renowned for their central bioenergetic role in eukaryotic cells, where they act as powerhouses to generate adenosine.
Principles of mitochondrial biology, metabolism and. Diverse dietary fat sources differently affect mitochondrial dynamics and bioenergetics. Written in the highly successful methods in molecular biology series format, chapters. Written in the highly successful methods in molecular biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents. Mar 30, 2020 mitochondrial dynamicsmitochondrial fission and. Mitochondrial fusion is an essential process for preserving the integrity and stability of mitochondrial dna. Bioenergetics is a field in biochemistry and cell biology that concerns energy flow through living systems. Details cellular bioenergetics, mitochondrial cell biology and signal transduction, and the roles of mitochondria in physiology, disease and aging.
Mitochondrial dynamics in neurodegeneration aims press. May 23, 2001 this title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. We are also examining the role of posttranslational modification in the function of mitochondrial proteins. Network morphology has been linked with the energy state in different cell types. Bioenergetic dysfunction occurs in alzheimers disease ad and mild. Noteworthy, emerging findings revealed an important role for mitochondrial dynamics within neuronal populations involved in central regulation of body energy balance. An uncoupling channel within the csubunit of the atp synthase is the permeability transition pore. Mar 22, 2017 in this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Mitochondrial fissionfusion dynamics and mitochondrial bioenergetics, including oxidative phosphorylation and generation of atp, are strongly clock controlled. Mitochondrial dynamics in the regulation of nutrient utilization and. Mitochondrial fissionfusion dynamics are also altered in ad and.
This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Biophysics summary bioenergetics 2 aims to clarify topics such as the thermodynamics of bioenergetic processes and the stoichiometries of energy coupling reactions. Biochimica et biophysica acta bioenergetics journal. Mitochondrial bioenergetics, redox state, dynamics and turnover alterations in renal mass reduction models of chronic kidney diseases and their possible implications in the progression of this illness. Sep 29, 2017 working model of the impact of 4hne on neuronal autophagy, mitochondrial dynamics and bioenergetics. To this end we assess oxygen consumption of cells, permeabilized cells or isolated mitochondria. Bioenergetic flux, mitochondrial mass and mitochondrial. The chapters in this book explore topics such as highresolution fluorespirometry and oxphos protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Mitochondrial bioenergetics and quality control mechanisms in health and disease a special issue journal published by hindawi since the first endosymbiotic event occurred, where a proteobacterium was engulfed by larger cells, evolutionary pressure was imposed into mitochondria in order to facilitate the higher energy output required for the. Thirty years of progress in mitochondrial bioenergetics. Pdf diet impact on mitochondrial bioenergetics and dynamics. Cardiolipin binding sites in mitochondrial proteins bound permanently to speci.
Biochimica et biophysica acta bioenergetics editorial board. Purchase thirty years of progress in mitochondrial bioenergetics and molecular biology 1st edition. The lab is equipped with last generation oroboros oxygraph o2k instruments, fluorimeter and spectrophotometer allowing proper and accurate characterization of cellular and mitochondrial bioenergetics properties. This course will give an overview of mitochondrial biology with a slant towards the clinical spectrum of human diseases affected by dysfunctional mitochondria. Pdf in this chapter we describe the fundamental mechanisms by which. The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. This phenomenon, known as mitochondrial dynamics, represents a key quality control system to maintain a healthy mitochondrial population but also a mechanism to bioenergetically adapt to the cellular and tissue energetic demands. We present new data on mitochondrial dynamics and dysfunction to illustrate a causal link between mitochondrial dna defects, excessive fission, mitochondrial retrograde signaling and cancer progression. All these clinical manifestations arise from the central role of bioenergetics in cell biology. Circadian control of drp1 activity regulates mitochondrial. Impaired mitochondrial dynamics and bioenergetics in diabetic skeletal muscle ruohai liu1, pengpeng jin2, liqunyu2, ying wang2, liping han2, tong shi1,xuli2 1department of anaesthesia, second affiliated hospital, wenzhou medical university, wenzhou, china, 2department of physiology, renji college, wenzhou medical.
Contrary to the textbook view of mitochondria as static rod shaped organelles. We have several tools allowing visualising and characterizing mitochondrial morphology and evaluating mitochondrial dynamic activity. The metabolic signals that link mitochondrial structure to its functional states have not been fully characterized. Even in the midst of conceptual and technical revolutions, bioenergeticists typically. Metabolic reprogramming universally occurs in cancer. Volume 83, issue 9, pages a1a14, e47e49, 717790 1 may 2018. Mitochondrial bioenergetics school of medical sciences. Mitochondria are renowned for their central bioenergetic role in eukaryotic cells, where they act as powerhouses to generate adenosine triphosphate from oxidation of nutrients.
Chapter 9 optimisation of cell bioenergetics in foodassociated microorganisms 219 diego mora and stefania arioli part 3 new techniques and findings in bioenergetics research 235 chapter 10 phosphorescence oxygen analyzer as a measuring tool for cellular bioenergetics 237 fatma aljasmi, ahmed r. This is the first book that integrates in one volume such diverse subjects as the genetic control of mitochondrial dynamics, the relationship between mitochondrial dynamics and bioenergetics, the roles of mitochondrial dynamics in apoptosis, axonal transport, mitochondrial quality control, and the contribution of defective mitochondrial. At the same time, these organelles are highly dynamic and undergo fusion, fission, transport, and degradation. Mitochondrial bioenergetics methods and protocols carlos m.
Mitochondrial bioenergetics and cardiolipin alterations in. This is an active area of biological research that includes the study of the transformation of energy in living organisms and the study of thousands of different cellular processes such as cellular respiration and the many other metabolic and enzymatic processes that lead to production. Fusion and fission is to discuss recent developments in mitochondrial dynamics in aging, cancer, cardiovascular, diabetesobesity, agerelated neurodegenerative diseases, and inherited mitochondrial diseases. In conclusion, mitochondrial dynamic processes with their strict interconnection with mitochondrial bioenergetics are involved in energy balance and diet impact on metabolic tissues.
Impaired mitochondrial dynamics and bioenergetics in. It is now accepted that this process of mitochondrial dynamics encompasses four different, but interconnected pathways figure 1. Bioenergetic role of mitochondrial fusion and fission sciencedirect. Diet impact on mitochondrial bioenergetics and dynamics. A graduate of moscow university, he was the longtime head of the laboratory of cardiac bioenergetics in moscow, russia, before taking up his present positions. The control of mitochondrial shape and size, two key aspects of mitochondrial. Jul 26, 2010 in addition, it would be useful to identify chemical compounds that could modify mitochondrial dynamics for research or therapeutic use. During the last decade, substantial empirical and in silico evidence was presented demonstrating that mitochondrial dynamics. Peter mitchell 19201992 metabolism in mitochondria. The cell biological aspect of mitochondria, especially mitochondrial dynamics, has drawn much attention through implications in human pathology, including neurological disorders and metabolic diseases. The equilibrium between mitochondrial fusionfission, as well as the organelle motility, determine network morphology and ultimately mitochondrial cell function. Antioxidants free fulltext a disturbance in the force.
Apr 17, 2012 the mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. Perturbed mitochondrial dynamics, an emerging aspect of. Principles of mitochondrial biology, metabolism and bioenergetics in health and disease mip spring, london 2015 a workshop to be held at university college london, april 20th24th monday april 20th tuesday april 21st wednesday april 22nd thursday april 23rd friday april 24th chair. Mitochondrial bioenergetics and structural network. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Direct substrate delivery into mitochondrial fission.
This article is part of a special issue entitled mitochondria in cancer, edited by giuseppe gasparre, rodrigue rossignol and pierre sonveaux. Metabolic regulation of mitochondrial dynamics journal of. Modeling mitochondrial bioenergetics with integrated. The book will include articles pertaining to mitochondrial fitness on a global basis, providing therefore an update on the progress made in several aspects in the field.
Each of these dynamic processes is critical for maintaining a healthy mitochondrial population. By acute pharmacological inhibition of mitochondrial fission protein drp1, we demonstrate in this study that mitochondrial fission is necessary for glucosestimulated insulin. Here we show that these circadian oscillations depend on circadian modification of dynaminrelated protein 1 drp1, a key mediator of mitochondrial fission. Another area of research is the importance of alterations in mitochondrial bioenergetics in certain types of cancers. Mitochondrial dynamics and metabolic regulation timothy wai1,2 and thomas langer1,2,3,4, mitochondrial morphology varies tremendously across cell types and tissues, changing rapidly in response to external insults and metabolic cues, such as nutrient status.
There is a unique set of appendices that include gene. Frap fluorescence recovery after photobleaching studies can be performed to understand mitochondrial dynamics or dynamics of any mitochondrial molecule within the organelle. Within cells, mitochondria are motile and display temporal changes in internal and external structure mitochondrial dynamics. Apr 08, 2015 noteworthy, emerging findings revealed an important role for mitochondrial dynamics within neuronal populations involved in central regulation of body energy balance. Drp1is involved in the fission of the mitochondrial outer membrane, perhaps directly or perhaps through its ability to recruit additional fission machinery components. Jci a mitochondrial bioenergetic etiology of disease. Mitochondrial bioenergetics and quality control mechanisms in.
Impairedmitochondrialdynamicsandbioenergeticsindiabeticskeletalmusclepone. Fragmented mitochondria are frequently found in resting cells, and mitochondrial fission plays an important role in the removal of damaged organelles by. These online bookshops told us they have this item. Three factors can perturb mitochondrial bioenergetics and result in disease. In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Regulation of autophagy, mitochondrial dynamics, and cellular. The book will include articles pertaining to mitochondrial fitness on a global basis, providing therefore an. Mitochondria form an interconnected and dynamic web that undergoes continuous cycles of fusion and fission events. The many functions of mitochondria have been intimately linked and. View the article pdf and any associated supplements and figures for a period of 48 hours.
Find out more about the editorial board for biochimica et biophysica acta bioenergetics. Jun 15, 2018 mitochondrial bioenergetics and quality control mechanisms in health and disease a special issue journal published by hindawi since the first endosymbiotic event occurred, where a proteobacterium was engulfed by larger cells, evolutionary pressure was imposed into mitochondria in order to facilitate the higher energy output required for the. Precisely how exercise regulates the network of these responses and their effects on mitochondrial dynamics is not fully understood at present. Pdf monitoring the mitochondrial dynamics in mammalian cells. It will introduce students to basic mitochondrial biology, such as structure and function, genetics and bioenergetics. Mitochondria form a dynamic network, and it remains unclear how the alternate configurations interact with bioenergetics properties.
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